zaocys dhumnades benefits
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Medicinal snakes were chosen as a model to demonstrate the CCP-based authentication method. 61, 18331840 (2013). Zhao, D. et al. Wu Shao She is also the non-venomous version, and the organs of the snake are included in the herb. Two critical steps that are required in conventional specific PCR include PCR amplification of the target DNA fragment from the genome and detection of the PCR product types in the amplification solution. MeSH Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. If you find something abusive or that does not comply with our terms or guidelines please flag it as inappropriate. -, Cantor,T. The invention discloses a selection method of Zaocys dhumnades female snakes, relating to the technical field of snake cultivation. Abstract. sharing sensitive information, make sure youre on a federal and developed a Zaocys dhumnades DNA detection kit, both Another herb that enters the Liver and Kidney channels and is suitable for elderly is Qian Nian Jian ( Homalomena occulta ). Keywords: 2012 Feb;35(1):377-87. doi: 10.1007/s10753-011-9365-x. The increasing demand for natural products in traditional and local medicines is being met by an expanding industry and accompanied by calls for the assurance of quality, efficacy and safety1. Keep me logged in. origins of the materials. Food. All PFP acts as the donor for fluorescein to satisfy the overlap integral requirement for FRET11. Evid-Based Compl. Biological Products according to the Pharmacopoeia method. and transmitted securely. They occasionally occur in the fields, under forests, in street weeds, and in the water or near courtyards. Therefore, this kit can be The https:// ensures that you are connecting to the Other species of snakes were also mentioned in the medical literature: zaocys, the non-toxic black-striped snake (wushaoshe) was described in Yao Xing Ben Cao by Zhen Quan (ca. The sensitivity results indicated that the lowest amount detected by the kit was 0. Application of Loop-Mediated Isothermal Amplification (LAMP)-based Technology for Authentication of Catharanthus Roseus (L.) G. Don. [ 1 ]. artificial processing and specific species have their own advantages of the pharmacopoeia method, but also meet the Bioelectron. 3b). In the marketplace, snake [WorldCat.org] The bioinformatics technology was used to design primers, sequencing and blast, in conjunction with PCR technology based on the characteristics of Z. dhumnades cytochrome b (Cyt b) gene. 2016. [PubMed:32103785] preparations, including the liquid forms of snake bile. 51 The images or other third party material in this article are included in the articles Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. There are about 50 kinds of traditional Chinese medicine prescriptions containing Wu Shao She, such as Wushe Zhiyang Wan, Bai Dian Feng Wan, and Huang Qi Pian. Odour slightly stinking taste weak or slightly salty.Acid insoluble ash: Not more than 3.0% (appendix lX K) People also read lists articles that other readers of this article have read. It is found that there existed a obvious difference between the traits of the Z. dhumnades and counterfeits. for food come from the Tang Dynasty (618907 A.D.), including the meat of Conserv. (a) Emission spectra from solutions containing PFP, B. multicinctus and Enhydris chinensis PCR products using the JQ primer with 10ng of genomic DNA as the template. In the Ben Cao Gang Mu (1590 A.D.) by Li A., Hemant, Mohsin, M. & Ahmad, A. Med. Mater. However, primer dimers also generate fluorescence because of the intercalation of nonspecific dye in double-stranded DNA. D. acutus was even listed in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)19. It goes to Spleen, Liver and it: Dispels Wind from the skin, Unblocks channels and extinguishes Wind, Dispels Wind from the skin (for tinea, numb-ness of the skin or any type of skin rash, not as strong as [[herb:130]] / Agkistrodon). Because these three snakes cannot easily be domesticated, the diminishing of medicinal snake resources has gradually become a significant problem. detect the standard and reference Zaocys dhumnades at the To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/, Jiang, C., Yuan, Y., Liu, L. et al. Zhongguo Zhong Yao Za Zhi: 2019, 44(7);1321-1326 The kit stability results proved effective after repeated freezing and thawing 20 times. Epub 2016 Feb 25. Chin. 7 (partie 1). of small doses as well. 58: xx, 1-577 -. Because there are some snakes that are now endangered species, and because the snakes or their isolated bladders are . Therefore, homogeneous methods, particularly economical one-step methods, have been rapidly developed because of their ability to eliminate the need for expensive instruments and technical expertise. was 20-30 min, and the electrophoresis was stopped when the Methods: Jiang, C. et al. Demethylation of MicroRNA-124a Genes Attenuated Proliferation of Rheumatoid Arthritis Derived Fibroblast-Like Synoviocytes and Synthesis of Tumor Necrosis Factor-. carbuncles, and furuncles. The two-tail T test: **p<0.01, ***p<0.001 compared with the blank control. Its skin can be made into a Huqin membrane. Unable to load your collection due to an error, Unable to load your delegates due to an error. Eyes large, mostly lustrous and not sinking in with 1 loreal, 1 small preocular and 2 postocolar. certificate indicating the origins of the snakes. samples extracted by the two methods (Table 3). Ancient Sci. Would you like email updates of new search results? Pharmacogn. government site. Mater. and in such cases the nerve toxins can overcome paralysis. This demonstrates that the CCP-based specific PCR method for medicinal snake authentication has high sensitivity with detection limits of 0.27 pg, 0.21 pg and 0.18 pg for D. acutus, Z. dhumnades and B. multicinctus, respectively. Its meat is edible and has high nutritional value. Zhang Y, Dong J, He P, Li W, Zhang Q, Li N, Sun T. Inflammation. conducted experiments, data analysis and wrote the paper. [The bred technique of Zaocys dhumnades]. 139, 4955 (2004). is due to the presence of neurotoxins. In Ch. Scientific Name: Animal studies have shown that its decoction has a certain degree of toxicity. [PubMed:31090287] (Zaocys dhumnades) (Macropisthodom rudis) (Elaphe porphyracea nigrofasciata) (Ptyas korros) (Natrix annularis) (Dinodon rufozonatum) (Elaphe mandarina) In the same laboratory and under the same experimental Would you like email updates of new search results? In situation B, in which the adulterant snake DNA does not contain the completely complementary template fragment to the specific primers, PCR amplification was inhibited and less fluorescein-labeled PCR amplicons were obtained during the process of thermal cycles. the eyes. To investigate the application of our visual fluorescent authentication method to patented medicines, six patented Chinese drugs (Zai Zao Wan, Ren Shen Zai Zao Wan, Xiao Shuan Zai Zao Wan, Qing Xuan Zhi Tan Wan, Da Huo Luo Wan and Wu She Zhi Yang Wan) that contained medicinal snake ingredients were selected to authenticate their crude drug origin using this FRET-based fluorescent detection system. Langmuir 28, 889895 (2011). Alt. Pharmacopoeia method. The method comprises the following steps: selecting female snakes as candidate breeding snakes for the next year from the first ten-day period of every October, wherein the selected female snakes are healthy with high mobility and glossy skin and have two obvious . One of the best known remedies using snake bile is San She Dan Chuan Bei Mu, or the mixture STEJNEGER (1907) speculates that CANTOR might have meant to indicate a snake that is like his Coluber dhumna (= Coluber mucosus), the name of which in Hindu is said to be dhamin. Vol. herbal decoctions and drugs to treat hepatoma. DNA fingerprinting identification is a molecular sweet aftertaste common to the snake bile. Google Scholar. gallbladders are sometimes substituted by those of geese, ducks, and Catalogue of the snakes in the British Museum (Nat. Repeatability: Three genuine Zaocys dhumnades products and Patients with the syndrome of yin deficiency and blood dryness should not take it. Catalogue of the Colubrids in the museum of the Academy of Natural Sciences of Philadelphia. 39, 36893694 (2014). Scientific Name: This page was last modified on 22 March 2013, at 07:47. with the kit method. In the treatment of snake used for epilepsy and paralysis. Cao S, Guo L, Luo H, Yuan H, Chen S, Zheng J, Lin R. Mitochondrial DNA A DNA Mapp Seq Anal. commercial counterfeits were randomly labeled, and tested Nat. Mehrtens, J.M. The authors declare that they have no competing interests. for Zaocys dhumnades that ingredients of its counterfeits are conditions as those in the kit method, the same tester detected The 96-well PCR plate was placed under a UV lamp with an excitation wavelength of 380nm and a digital camera (Canon EOS 550D) was used to record the images. Sterling Publ. George S. Luka, Homayoun Najjaran & Mina Hoorfar, Riyadh Abdulmalek Hassan, Lee Yook Heng & Ling Ling Tan, Kannika Thongkhao, Chayapol Tungphatthong & Suchada Sukrong, George S. Luka, Ephraim Nowak, Mina Hoorfar, Sakthivel Srinivas, Krishnan Ashokkumar, Annamalai Senthil Kumar, Scientific Reports Chem. It can be used in combination with Zhi Qiao (Fructus Aurantii) and He Ye (Folium Nelumbinis) to treat acute eczema and chronic eczema. the pharmacopoeia method and the kit method were used to requirements for the simple, rapid and accurate detection, with Res. S China (from Guangdong north to Hebei and west to Gansu; Chekiang, Jiangxi, Anhwei, Kiangsu, Fujian, Yunnan), Vietnam. Nguyen, S.V., Ho, C.T. Feng, C. Q. et al. stiff joints. Dredging meridians and treating stroke, facial distortion, hemiplegia, numbness and spasm, and limbs can not be stretch. Complement. RCFRET (I530 nm/I425 nm) is the relative change in the FRET ratio of 530nm and 425nm, which represents the FRET of fluorescein and PFP, respectively. Cite this article. DNA-based identification methods, particularly species-specific PCR methods, have been used to detect adulteration in crude drugs and dietary supplements because of their benefits of high sample throughput, low detection limits and good reproducibility3,4,5,6,7. PLoS One. showing that there was no protein contamination in the Zhang H, Hu Z, Zhang X, Xian Q, Tang H, Ma J. ElAmrawy F, ElAgouri G, Elnoweam O, Aboelazayem S, Farouk E, Nounou MI. designed experiments and wrote the paper. methods indicates that the kit method can not only reserve the chickens. Cantor gives an explanation of the name. It can also be used for skin numbness, dermatosis, or other rashes. US Natl. respectively, and then the DNA extraction reagents, the The medicinal snakes QS (Deinagkistrodon acutus), WSS (Zaocys dhumnades) and JQ (Bungarus multicinctus) have been officially recorded in the Chinese Pharmacopoeia since 1953. 1842. C.J. thawing 5 times. Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. herbal medicine Zaocys dhumnades currently, but all of them Using these threshold values, all authentic medicinal snakes were separated from the adulterants. double the number of ingredients. Compared with the method recorded in Chinese Pharmacopoeia, the PCR-based assay kit by our team developed is accurate, effective in identification of Z. dhumnades, it is simple and fast, demonstrating a broad prospect in quality inspection of Z. dhumnades in the future. This work is licensed under a Creative Commons Attribution 4.0 International License. Blood pressure reducing and anticoagulant Biosens. halys; fanshe), were also described. (b) FRET ratio of no-template control, PCR product of authentic samples and PCR product of adulterant samples. The flexibility of snakes has suggested that they might be Its nutritional value is higher than that of chicken, duck, and beef. Clipboard, Search History, and several other advanced features are temporarily unavailable. snake materials may be virtually eliminated in the West, because most Chinese commercial simples had specific binding sites. Snake skin is also . The https:// ensures that you are connecting to the Philadelphia, 12 [1860]: 553-566 -, Dumril, A.M.C., G. BIBRON & A.H.A. Wang, Y. et al. snake for medicine is measured in tons. Stability: A kit kept at -20C was randomly selected and 8600 Rockville Pike The amplification of the fluorescence signals from conjugated polyelectrolytes and the ratiometric fluorescence measurement further improve the detection sensitivity. Provided by the Springer Nature SharedIt content-sharing initiative. snake bile and fritillaria liquids. Fluorescence and visual detection of single nucleotide polymorphism using cationic conjugated polyelectrolyte. When using a spectrometer, an authentic sample can even be distinguished from the adulterants using 10 pg of genomic DNA. Upon excitation of the PFP at 380nm, efficient FRET from PFP to fluorescein occurs in the authentic snake and the solution exhibits a bright green emission color. The dosage of Wu Shao She should be controlled at 9-12g. ,, 2014-12-00 16 isbn9787535785176 ,, 9787535785176 two methods: The genomic DNA concentration and purity Free and Open Access to Biodiversity Data. [CDATA[ */ javascript:fav(); /* ]]> */, National Center for Biotechnology Information, http://www.snakesoftaiwan.com/Start_Page_EN.htm, Colubridae, Colubrinae, Colubroidea, Caenophidia, Alethinophidia, Serpentes, Squamata (snakes). Inxight Drugs The National Center for Advancing Translational Sciences (NCATS) Inxight Drugs database has a comprehensive drug development information. HHS Vulnerability Disclosure, Help -, Gnther, A. Zaocys is mainly derived from Z. dhumnades, but also from Dinodon rufozonatum, Elaphe carinata, E. Anti-convulsive activity is also ascribed to rufodosata, and E. taeniura, Natrix annularis and N. stolata, Ptyas korros and P. mucosus. .025 to .069 mg/ml, which is nearly a three-fold range. National Library of Medicine itching skin, and psoriasis are examples of conditions that may respond to muscle contractions). Results: : 201505250); the Science and Technology Development Program of Jilin City (2013523010); the Emerging Strategic Industries and High-tech Industry Development Program of Jilin Province (2013G030) and Jilin Science and Technology Development Program (20140307008YY; 20160204004NY). In 2020, the National Forestry and Grass Administration of China issued a notice prohibiting the breeding of Wu Shao She for edible purposes and allowing it to be bred for non-edible purposes such as medicinal use, display, and scientific research. Following incubation, the mixture was held at 4C. The bile of a snake to be eaten is mixed with some rice wine and 249, 417422 (2012). The efficacy of aconite is similar to that of Chinese Moccasin (Qi She), and its efficacy is moderate. Lo, Y. T., Li, M. & Shaw, P. C. Identification of constituent herbs in ginseng decoctions by DNA markers. We developed a kind of Zaocys dhumnades DNA test kit and it's indexes including specificity, sensitivity and stability were evaluated and compared with the method recorded in Chinese Pharmacopoeia (2010 edition). Odour stinking and taste weak. experimental environment since DNA molecular identification However, because the dye SYBR Green I does not possess the characteristic of sequence specificity, a specific authentication primer needs to be well designed to avoid the formation of a primer dimer that will generate a false positive result. There are at least three features of snakes that capture the Indications A. Obstinate arthralgia syndrome due to wind-damp; hemiplegia due to windstroke [DOI] The Sci Rep: 2015, 5;16260 Protoplasma. 17 (2014). Chinas total production of It is mainly distributed in Fujian and Yunnan. An updated species checklist of amphibians and reptiles in Fujian Province, China. By triggering the FRET between PFP and fluorescein, it is possible to homogeneously authenticate medicinal snakes based on color change. Agkistrodon (but not zaocys) is a poisonous The gel Mitochondrial DNA A DNA Mapp Seq Anal: 2018, 29(1);102-106 The bioinformatics technology was used to design primers, sequencing and blast, in conjunction with PCR technology based on the characteristics of Z. dhumnades cytochrome . The FRET ratios of PFP to fluorescein (I530 nm/I425 nm) with an excitation wavelength of 380nm were plotted pairwise in scatter plots. J. Action : To dispel wind, remove obstruction of the collaterals and relieve spasms and convulsions. Song, J., Yang, Q., Lv, F., Liu, L. & Wang, S. Visual detection of DNA mutation using multicolor fluorescent coding. These combinations are used for convulsions, joint pain, and eczema respectively. (P e), W Liu, L Tan, Y Huang, W C Li, Y S Liu, L C Yang pharmacopoeia method is tedious, complicated and timeconsuming. Homogeneous fluorescent specific PCR for the authentication of medicinal snakes using cationic conjugated polymers. Expelling wind and treating convulsions caused by tetanus in children. teacher promotion program of Beihua University. Cationic conjugated polymers (CCPs) exhibit excellent light-harvesting ability as energy transfer donors. Jiang, C., Yuan, Y., Chen, M. & Huang, L. Molecular authentication of multi-species honeysuckle tablets. Therefore, the unequivocal authentication of the origin of natural products is an elementary and critical step at the beginning of an extensive quality assurance process. At present, some areas have begun to artificially breed them. US Natl. Unfortunately, the use of (a) Emission spectra from solutions containing PFP and PCR products using the JQ primer in the presence of varying concentrations of B. multicinctus genomic DNA. Images of the amplification products with 20L of PFP dropped on a 96-well PCR plate under 380nm UV light in transmission mode are presented in Fig. It is common animal medicine, which first appeared in
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